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首页> 外文OA文献 >The application of primary trisomics and transposon-induced mutations in genetic studies of soybean (Glycine max (L.) Merr)
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The application of primary trisomics and transposon-induced mutations in genetic studies of soybean (Glycine max (L.) Merr)

机译：三体组学和转座子诱导的突变在大豆遗传研究中的应用（Glycine max（L.）Merr）

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摘要

The development of a soybean (Glycine max (L.) Merrill) genetic map is an ongoing process. Formulae to calculate expected critical genotypic and phenotypic F[subscript]2 ratios were developed to facilitate the mapping of codominant markers by using primary trisomics. A method is given for calculating the minimum family size required to distinguish between a critical and noncritical segregation ratio when three phenotypic classes are present;The five known soybean primary trisomics A, B, C, D, and S were tested for linkage with 10 isozyme loci. Only one locus was assigned to a chromosome. The Dial (diaphorase) locus was located on the extra chromosome in Trisomic D;By analyzing segregating F[subscript]2 populations. the isozyme marker, Idh1 (isocitrate dehydrogenase), was mapped to linkage group 11. The gene order on this linkage group was F-Idh1-Rj1. In a similar study, a new linkage group was found. Pgi2 (phosphoglucoisomerase) was linked to the W4 locus with a distance of 44.8 ± 2.2 centimorgans;An independent area of research was the analysis of three different chlorophyll-deficient mutants (CD) derived from the w4-m soybean line that contains a transposable element. The w4-m line has flowers with purple sectors on a near-white background. Each of the CDs were a pale-green color and lacked the same two mitochondrial malate dehydrogenase enzymes. Allelism tests indicated each of the CDs contained a mutation in the same chromosomal region. The CD mutants and T253 (y20-k2) from the Soybean Genetic Type Collection were allelic. The yellow-green phenotype and the MDH-null were inherited as single recessive alleles. The plant color was stable even when the plant had mutable flowers. Therefore, neither a receptor nor a regulatory element was producing the mutant phenotypes. The CD mutants probably contain deletions involving the Y20 and MDH loci. The different phenotypes of the CD mutants suggest that the deletions have different endpoints. The deletions were not large enough to affect the transmission of the aberrant chromosome through the male and female gametes.
机译：大豆（Glycine max（L.）Merrill）遗传图谱的开发是一个持续的过程。开发了用于计算预期的关键基因型和表型F 2比率的公式，以利于通过使用初级三体组学绘制共性标记。当存在三种表型类别时，给出了一种计算最小家族大小以区分临界和非临界分离比的方法；测试了五个已知的大豆初级三体组A，B，C，D和S与10种同工酶的连接性位点。仅一个基因座被分配给一条染色体。 Dial（心肌酶）基因座位于Trisomic D的额外染色体上；通过分析分离的F [subscript] 2种群。将同工酶标记Idh1（异柠檬酸脱氢酶）定位到连接基团11。该连接基团上的基因顺序为F-Idh1-Rj1。在类似的研究中，发现了一个新的联系小组。 Pgi2（磷酸葡萄糖异构酶）与W4基因座的连锁距离为44.8±2.2厘摩；一个独立的研究领域是分析来自w4-m大豆品系的三个不同叶绿素缺陷型突变体（CD），其中含有可转座因子。 w4-m线的花朵接近白色背景上有紫色扇形。每张CD均为浅绿色，缺少相同的两种线粒体苹果酸脱氢酶。等位性测试表明，每张CD都在同一染色体区域内包含一个突变。来自大豆遗传类型收集中心的CD突变体和T253（y20-k2）是等位基因。黄绿色表型和MDH-null被遗传为单个隐性等位基因。即使植物具有可变的花朵，植物的颜色也是稳定的。因此，受体和调节元件均未产生突变表型。 CD突变体可能包含涉及Y20和MDH基因座的缺失。 CD突变体的不同表型表明缺失具有不同的终点。缺失不足以影响异常染色体通过雄配子和雌配子的传递。

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  • 作者

    Hedges, Bradley Robert;

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  • 年度 1989
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  • 正文语种 en
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